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Palmitoyl Tripeptide-1

low risk

Also: Pal-GHK · Palmitoyl-GHK · Matrixyl component · Biopeptide CL

Moderate Evidence Research Only

Palmitoyl Tripeptide-1 (Pal-GHK) is a fatty acid-conjugated peptide derived from the collagen breakdown sequence Gly-His-Lys (GHK). The palmitoyl group enhances skin penetration by increasing lipophilicity. It is a key component of Matrixyl (combined with Palmitoyl Pentapeptide-4) and is one of the most widely used cosmetic active ingredients. In vitro and limited clinical data support its role in stimulating collagen I and III synthesis, fibronectin, and glycosaminoglycans in dermal fibroblasts.

Molecular Weight
594.8 Da
Formula
C30H54N4O5S
Common Dosing
Topical use only. Typical concentration in formulations: 0.0001%–0.01% (1–10 ppm). Applied once or twice daily as part of a serum or moisturizer.
Category
cosmetic
Last Reviewed
2026-05-25

Reported Benefits

Collagen synthesis and skin firmness

Moderate Evidence 24 studies

In vitro studies consistently show increased collagen I, III, and fibronectin production in fibroblasts treated with Pal-GHK. Small clinical studies demonstrate measurable wrinkle reduction and skin firmness improvements.

Wrinkle reduction

Moderate Evidence 17 studies

Clinical evaluations of Matrixyl (which contains Pal-GHK) showed statistically significant wrinkle reduction vs. placebo in 12-week studies, including the widely cited Levin and Momin review.

Wound healing support

Preliminary 8 studies

GHK and its derivatives promote wound contraction and re-epithelialization in animal models; topical cosmetic concentrations are below therapeutic wound-healing levels.

Mechanism of Action

GHK is an endogenous copper-binding tripeptide generated during collagen breakdown. It signals to fibroblasts to upregulate extracellular matrix production (collagen I, III, fibronectin, glycosaminoglycans) and downregulate matrix metalloproteinases (MMP-1, MMP-2, MMP-9). The palmitoyl modification facilitates percutaneous absorption through the stratum corneum by increasing lipid bilayer partitioning. Palmitoyl Tripeptide-1 mimics this matrikine signaling.

Key Clinical Studies

Katayama et al. (1993)

Randomized, double-blind, split-face · 12

PubMed →

Palmitoyl pentapeptide-4 (close analog) reduced wrinkle volume and skin roughness; supported mechanism for Pal-GHK class

Robinson et al. (2005)

Randomized, double-blind, placebo-controlled · 93

PubMed →

Matrixyl formulation (containing Pal-GHK and Pal-KTTKS) significantly reduced wrinkle depth vs. placebo at 12 weeks

Overview

Palmitoyl Tripeptide-1 is a lipidated matrikine peptide — a fragment of collagen’s breakdown products conjugated to a fatty acid to improve skin penetration. Its active sequence, GHK (Gly-His-Lys), is the same found in the copper-binding peptide GHK-Cu, though Palmitoyl Tripeptide-1 does not bind copper and works through a different delivery and signaling mechanism. It is most commonly known as one half of Matrixyl, a widely used cosmetic ingredient combination developed by Sederma.

Evidence Summary

The evidence for Palmitoyl Tripeptide-1 is more solid than most cosmetic peptides. The in vitro data is consistent across labs, and several small but controlled clinical trials support wrinkle reduction and skin firmness improvements at typical formulation concentrations. The limitations: most clinical studies are industry-sponsored, sample sizes are small (30–100 subjects), and study duration rarely exceeds 12 weeks. Long-term comparative data against retinoids or established actives is absent. The effect size is real but modest.

Regulatory Status

Palmitoyl Tripeptide-1 is a cosmetic ingredient — not a drug. It is not FDA-regulated beyond general cosmetic safety requirements. No prescription required. Listed on the CosIng database (EU cosmetics inventory) with documented safety profile. INCI name: Palmitoyl Tripeptide-1.

Most Common Use Cases

Used in anti-aging serums, neck creams, eye area treatments, and moisturizers targeting fine lines and loss of firmness. Most effective in well-formulated products at concentrations above 5 ppm, combined with skin barrier support ingredients. Often paired with Palmitoyl Pentapeptide-4 (Matrixyl) or Palmitoyl Tripeptide-5 for complementary collagen-stimulating effects.

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Nava Health

Regulatory Status

Research Only

Safety Profile

Side Effects

  • Generally well tolerated
  • Rare: contact sensitization in hypersensitive individuals

Contraindications

  • Known hypersensitivity to palmitic acid or GHK sequence

Drug Interactions

  • None established at cosmetic use concentrations

Primary Uses

Collagen synthesis stimulationAnti-wrinkleSkin firmnessWound healing (topical)

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Disclaimer: This information is for educational and research purposes only. Not medical advice. Consult a qualified healthcare provider before using any compound.